A pair of primers , based on the high homologous n - and c - terminal amino acids sequences of subtilisin of bacillus pumilus , were designed and used to amplify subtilisin gene from genomic dna of 4 different bacillus strains by polymerase chain reaction Sds 、 edta和pmsf對酶活力有不同程度的抑制作用。以4株芽孢桿菌的總dna為模版,利用擴增短小芽孢桿菌堿性蛋白酶基因的引物對它們進行pcr擴增。
Many scholars think they have not important physical roles because of their high working concentration . they have the same n - terminal amino acid and c - terminal amino acid , namely asparine and cysteine respectively . they form two largely the same hydrophobic domains Bsp - a1 a2和a3的n -端都是天冬氨酸, c -端為半胱氨酸,并都含有兩對二硫鍵,由此形成了兩個基本相同的疏水結構區。